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KMID : 1149220180260040329
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Korean Journal of Oriental Medical Prescription
2018 Volume.26 No. 4 p.329 ~ p.340
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Hepato-protective Effects of Daucus carota L. Root Ethanol Extract through Activation of AMPK in HepG2 Cells
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Kim Do-Yeon
Park Sang-Mi
Byun Sung-Hee
Park Chung-A
Cho Il-Je
Kim Sang-Chan
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Abstract
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Objectives : In Traditional Korean medicine, Daucus carota L. has been used for treating dyspepsia, diarrhea, dysentery and cough. Recent pharmacognosic evidence showed D. carota has anti-oxidant, anti-cancer, anti-fungal, and hypotensive effects. Present study investigated hepato-protective effect of D. carota ethanol extract (DCE) against oxidative stress in HepG2 cells.
Methods : After HepG2 cells were pretreated with different concentrations of DCE, the cells were exposed to tert-butyl hydroperoxide (tBHP) for inducing oxidative stress. Cell viability, hydrogen peroxide production, glutathione concentration, and mitochondrial membrane potentials were measured to explore hepato-protective effect of DCE. Phosphorylation of AMP-activated protein kinase (AMPK) and effect of compound C on cell viability were determined to investigate the role of AMPK on DCE-mediated cytoprotection.
Results : DCE significantly decreased the tBHP-mediated cytotoxicity in a concentration dependent manner and reduced the changes on apoptosis-related proteins by tBHP in HepG2 cells. In addition, DCE significantly prevented hydrogen peroxide production, glutathione depletion, and mitochondrial membrane impairment induced by tBHP. Treatment with DCE increased phosphorylation of AMPK, and the DCE-mediated cytoprotection was abolished by pretreatment with compound C.
Conclusions : These results demonstrate that DCE can protect hepatocytes from oxidative stress through activation of AMPK.
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KEYWORD
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AMP-activated protein kinase (AMPK), Daucus carota L. ethanol extract (DCE), HepG2 cells, Oxidative stress, tert-butyl hydroperoxide (tBHP)
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